Enterotoxigenicity of Salmonella Gallinarum strains isolated from poultry

Twenty Salmonella Gallinarum strains isolated from poultry were tested for Enterotoxigenicity activity using rabbit and chicken ligated intestine loop technique and the rabbit vascular permeability factor activity test. One ml of cell-free filtrate (CFF) of each isolate was injected into chicken ligated loops and rabbit ligated loops, one ml of whole cell culture(WCC) of each isolate was injected into chicken ligated loops and 0.1 ml of crude enterotoxin was injected intermally in rabbit skin. Enterotoxigenic activity measured as mean dilatation index (DI) produced by Salmonella Gallinarum enterotoxins in rabbit loop test was significantly lower than that in the chicken intestine loop (p < o.o5) and did not differ significantly than the mean DI of WCC in the chicken intestine segment (p > 0.05). The filtrates from Salmonella Gallinarum strains gave permeability factor in 41.6 % of the cases. These data seem to support the concept of specificity of chicken intestinal loop method as suitable for detection of enterotoxigenicity of Salmonella Gallinarum.